Production of high-titer helper-free retroviruses by transient transfection.

  title={Production of high-titer helper-free retroviruses by transient transfection.},
  author={Warren S Pear and Garry P. Nolan and Martin L. Scott and David Baltimore},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={90 18},
The generation of high-titer, helper-free retroviruses by transient transfection has been achieved by using the highly transfectable 293T cell line into which are stably introduced constructs that express retroviral packaging functions. The resulting ecotropic virus packaging cell line BOSC 23 produces infectious retrovirus at > 10(6) infectious units/ml of supernatant within 72 hr after CaPO4-mediated transfection. A stringent assay for replication-competent virus showed that no helper virus… CONTINUE READING
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