Production of heterohybridomas secreting autoreactive and polyreactive human monoclonal antibodies.

@article{Snchez1992ProductionOH,
  title={Production of heterohybridomas secreting autoreactive and polyreactive human monoclonal antibodies.},
  author={B D{\'i}az S{\'a}nchez and Jos{\'e} Y{\'e}lamos and Jos{\'e} Melero and R Magari{\~n}o and Manuel Gonz{\'a}lez and Rosal{\'i}a Garc{\'i}a and Camilo R{\'i}os and Antonio N{\'u}{\~n}ez-Rold{\'a}n},
  journal={Scandinavian journal of immunology},
  year={1992},
  volume={35 1},
  pages={33-41}
}
Peripheral blood lymphocytes from two polytransfused renal dialysis patients were transformed by Epstein-Barr virus, fused to a heteromyeloma and cloned. Eight human monoclonal antibodies from the resulting clones were tested for their binding to a variety of antigens by ELISA, indirect immunofluorescence and immunoblotting. Antigens tested included B-cell lines, T and B lymphocytes, red blood cells, chronic lymphocytic leukaemic B cells, IgG, ssDNA, dsDNA, histones, nucleoprotamine, sperm… CONTINUE READING