Production of herpes simplex virus fluorescein labelled typing reagents.

  title={Production of herpes simplex virus fluorescein labelled typing reagents.},
  author={Paul Wilson and L M Cropper and Rudolf Patt and Ian R. Sharp},
  journal={Journal of virological methods},
  volume={45 1},
29 herpes simplex virus monoclonal antibodies (MAbs) were produced and shown by indirect immunofluorescence assay (IIFA) to be HSV type specific or HSV cross reactive. Two HSV-1 and two HSV-2 specific MAbs were selected as HSV typing reagents on the basis of their specificity and the fluorescence pattern produced. When directly conjugated to fluorescein both reagents detected and correctly identified HSV isolates previously typed by restriction endonuclease analysis (REA). No cross reactions… CONTINUE READING