• Corpus ID: 3889236

Production of herbicide-tolerant zoysiagrass by Agrobacterium-mediated transformation.

@article{Toyama2003ProductionOH,
  title={Production of herbicide-tolerant zoysiagrass by Agrobacterium-mediated transformation.},
  author={Koichi Toyama and Chang Hyu Bae and Joeng-Gu Kang and Yong Pyo Lim and Taiji Adachi and Key Zung Riu and Pill Soon Song and Hyo Yeon Lee},
  journal={Molecules and cells},
  year={2003},
  volume={16 1},
  pages={
          19-27
        }
}
Herbicide-resistant zoysiagrass (Zoysia japonica Steud.) has been developed by Agrobacterium-mediated transformation. A callus-type transformation system was established by optimizing several factors that affect the rate of transformation, including co-cultivation period and concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), CaCl2 and acetosyringone. Maximal GUS expression was observed when a Type 3 callus was co-cultivated on 2,4-D-free co-cultivation medium for 9 d. In addition… 

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