• Corpus ID: 3889236

Production of herbicide-tolerant zoysiagrass by Agrobacterium-mediated transformation.

  title={Production of herbicide-tolerant zoysiagrass by Agrobacterium-mediated transformation.},
  author={Koichi Toyama and Chang Hyu Bae and Joeng-Gu Kang and Yong Pyo Lim and Taiji Adachi and Key Zung Riu and Pill Soon Song and Hyo Yeon Lee},
  journal={Molecules and cells},
  volume={16 1},
Herbicide-resistant zoysiagrass (Zoysia japonica Steud.) has been developed by Agrobacterium-mediated transformation. A callus-type transformation system was established by optimizing several factors that affect the rate of transformation, including co-cultivation period and concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), CaCl2 and acetosyringone. Maximal GUS expression was observed when a Type 3 callus was co-cultivated on 2,4-D-free co-cultivation medium for 9 d. In addition… 

Figures and Tables from this paper

Transgenic zoysiagrass (Zoysia japonica) plants obtained by Agrobacterium-mediated transformation

A straightforward and efficient transformation protocol without callus induction and propagation is developed that opens up new opportunities for the functional characterization of genes and promoters and the development of novel germplasm in zoysiagrass.

Agrobacterium‐mediated transformation of Japanese lawngrass (Zoysia japonica Steud.) containing a synthetic cryIA(b) gene from Bacillus thuringiensis

Transgenic Japanese lawngrass plants containing a synthetic cryIA(b) gene from Bacillus thuringiensis under the control of a maize ubiquitin promoter were developed by Agrobacterium-mediated transformation by demonstrating that 59.1% of the transgenic events were inherited in a typical Mendelian fashion.

Agrobacterium-medlated high-efficiency transformation of creeping bentgrass with herbicide resistance

An efficient genetic transformation system for creeping bentgrass is established and a high frequency of single-copy transgene integration and functional gene expression is confirmed.

An Agrobacterium tumefaciens-mediated transformation system using callus of Zoysia tenuifolia Willd. ex Trin

This is the first report of successful plant regeneration and genetic transformation protocols for Z. tenuifolia using Agrobacterium tumefaciens and the transgenic nature of the transformants was demonstrated by the detection of β-glucuronidase activity in the primary transformants and by PCR and Southern hybridization analysis.

Effect of Surface Sterilization Method on Agrobacterium-mediated Transformation of Field-grown Zoysiagrass Stolon

An effective surface sterilization and culture method using the stolon explant for infection with Agrobacterium tumefaciens is developed and would speed up the development of transgenic plants by utilizing field-grown stolon nodes.

Transformation of recalcitrant turfgrass cultivars through improvement of tissue culture and selection regime

Chemical and molecular analyses confirmed that foreign genes were successfully introduced into the genome of perennial ryegrass or tall fescue.

Expression of CoQ10-producing ddsA transgene by efficient Agrobacterium-mediated transformation in Panicum meyerianum

The ddsA gene, which encodes decaprenyl diphosphate synthase and is required for coenzyme Q10 (CoQ10) synthesis, was transferred into P. meyerianum by using the optimized co-cultivation procedure for transformation and was confirmed by PCR and DNA gel blot hybridization that all hygromycin-resistant plants retained stable insertion of the hpt and dDSA genes.

Rapid and efficient callus induction and plant regeneration from seeds of zoysiagrass (Zoysia japonica Steud.)

With this system, it appears the duration of in vitro regeneration could be shortened to 16 weeks for zoysiagrass, i.e. from inoculation of embryos to regeneration of plantlets with shoots and roots.

Development of highly regenerable callus lines and Agrobacterium-mediated transformation of Chinese lawngrass (Zoysia sinica Hance) with a cold inducible transcription factor, CBF1

Five highly regenerable callus lines have been developed by addition of 6-benzylaminopurine and adjusting concentration of 2,4-dichlorophenoxyacetic acid in culture medium after multiple subculture, of which Zh44 was used for Agrobacterium-mediated transformation.

Agrobacterium-mediated transformed transgenic triploid bermudagrass (Cynodon dactylon × C. transvaalensis) plants are highly resistant to the glufosinate herbicide Liberty

Herbicide bioassay indicated that the bar-expressing transgenic plants exhibited greater herbicide resistance than the wild type and the non-transformed tissue culture-derived plants.



Agrobacterium-mediated genetic transformation of a phalaenopsis orchid

More than 100 hygromycin-resistant phalaenopsis plantlets were produced about 7 months following infection of the cell aggregates with Agrobacterium tumefaciens strains LBA4404 and EHA101 that harbored genes for β-glucuronidase (GUS) and hyGromycin resistance.

Agrobacterium-mediated transformation of citrange: factors affecting transformation and regeneration

The effects of cocultivation with Agrobacterium tumefaciens, regeneration and selection conditions on the transformation efficiency of citrange, including the number of regenerated escape shoots, have been investigated.

Genetic Transformation of Wheat Mediated by Agrobacterium tumefaciens

A rapid Agrobacterium tumefaciens-mediated transformation system for wheat was developed using freshly isolated immature embryos, precultured immature embryos and embryogenic calli as explants, and stable integration, expression, and inheritance of the transgenes were confirmed by molecular and genetic analysis.

Transgenic plant production mediated by Agrobacterium in Indica rice

A reproducible system has been developed for the production of transgenic plants in indica rice using Agrobacterium-mediated gene transfer and inclusion of acetosyringone in the Agrobacteria suspension and co-culture media proved to be indispensable for successful transformation.

Factors that influence Agrobacterium rhizogenes-mediated transformation of broccoli (Brassica oleracea L. var. italica)

An improved broccoli transformation system was developed by optimising several factors that affect the rate of effective Agrobacterium-mediated transformation, and produced transformation rates of 33% in preliminary experiments transforming broccoli with the antisense 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase gene from pTOM13.

Transgenic Japanese lawngrass (Zoysia japonica Steud.) plants regenerated from protoplasts

Transgenic Japanese lawngrass plants were generated by means of polyethylene glycol (PEG)-mediated direct gene transfer into protoplasts through the plasmid pBC1 and the gus gene regulated by the maize alcohol dehydrogenase-1 (Adh 1) promoter was expressed in the leaves and roots of transgenic Japanese Lawngrass plants.

Transformation of cucumber (Cucumis sativus L.) plants using Agrobacterium tumefaciens and regeneration from hypocotyl explants

Transgenic cucumber (Cucumis sativus L.) plants were successfully obtained from hypocotyl explants inoculated with Agrobacterium tumefaciens, which harbored a binary vector plasmid with NOS-nptII, CaMV 35S-I-gus and CaMS-hph genes, which enhanced the efficiency of transformation at the cut surface cells during five days of co-cultivation.

Sonication-assisted Agrobacterium-mediated transformation of soybean immature cotyledons: optimization of transient expression

Sonication-assisted Agrobacterium-mediated transformation (SAAT) tremendously improves the efficiency of Agrobacterial infection by introducing large numbers of microwounds into the target plant tissue by evaluating the effects of cultivars, binary vectors, optical density, and acetosyringone on transient β-glucuronidase activity.

Plant regeneration from protoplasts of Japanese lawngrass

Embryogenic callus of Japanese lawngrass was induced from sterile mature seeds on LS medium with 5 mg / l of 2,4-D and regenerated by transferring the protoplasts derived callus to MS medium and incubating at 28 °C under light for two months.