Production of glycerol from glucose by coexpressing glycerol-3-phosphate dehydrogenase and glycerol-3-phosphatase in Klebsiella pneumoniae.

@article{Zheng2008ProductionOG,
  title={Production of glycerol from glucose by coexpressing glycerol-3-phosphate dehydrogenase and glycerol-3-phosphatase in Klebsiella pneumoniae.},
  author={Yu Hui Zheng and Lu Zhao and Jianguo Zhang and Haiyi Zhang and Xingyuan Ma and Dongzhi Wei},
  journal={Journal of bioscience and bioengineering},
  year={2008},
  volume={105 5},
  pages={508-12}
}
As a valuable chemical, 1,3-propanediol (1,3-PD) could be biosynthesized by glycerol fermentation. However, no natural microorganisms that could directly convert glucose into 1,3-PD have been found so far. In this work, genes coding for two enzymes, glycerol-3-phosphate dehydrogenase (GPD, EC 1.1.1.8) and glycerol-3-phosphatase (GPP, EC 3.1.3.21), which were responsible for glycerol production, were organized into the plasmid pUC18K under control of the respective lac promoters. Two recombinant… CONTINUE READING

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References

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Showing 1-10 of 19 references

Evolution of a Saccharomyces cerevisiae metabolic pathway in Escherichia coli

I. Meynial Salles, N. Forchhammer, C. Croux, L. Girbal, P. Soucaillea
Metab. Eng., 9,152–159 • 2007

The effect of raw glycerol concentration on the production of 1,3-propanediol by Clostridium butyricum

S. Papanikolaou, M. Fick, G. Aggelis
J. Chem. Technol. Biotechnol., 79, 1189–1196 • 2004

Osmotic stress signaling and osmoadaptation in yeasts.

Microbiology and molecular biology reviews : MMBR • 2002

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