Production of germline chimera in loach (Misgurnus anguillicaudatus) and proposal of new method for preservation of endangered fish species.

@article{Nakagawa2002ProductionOG,
  title={Production of germline chimera in loach (Misgurnus anguillicaudatus) and proposal of new method for preservation of endangered fish species.},
  author={Masahiro Nakagawa and Toru Kobayashi and Koichi Ueno},
  journal={The Journal of experimental zoology},
  year={2002},
  volume={293 6},
  pages={624-31}
}
As part of the technique for preservation of endangered fish species, germline chimeras using the loach (Misgurnus anguillicaudatus) model were generated by embryonic cell manipulation. Transplanting cells from early-stage embryos of a wild-type strain to the blastoderm of an orange-type strain generated chimeras. Within three days, many of the resulting individuals exhibited a dark pigment on several parts of their body, characteristic of the donor but not of the orange strain. At one year of… CONTINUE READING

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