Production of gene-targeted sheep by nuclear transfer from cultured somatic cells

@article{McCreath2000ProductionOG,
  title={Production of gene-targeted sheep by nuclear transfer from cultured somatic cells},
  author={Kenneth J. McCreath and J. Howcroft and Keith Henry Stockman Campbell and Alan Colman and Angelika Schnieke and Alexander J. Kind},
  journal={Nature},
  year={2000},
  volume={405},
  pages={1066-1069}
}
It is over a decade since the first demonstration that mouse embryonic stem cells could be used to transfer a predetermined genetic modification to a whole animal. The extension of this technique to other mammalian species, particularly livestock, might bring numerous biomedical benefits, for example, ablation of xenoreactive transplantation antigens, inactivation of genes responsible for neuropathogenic disease and precise placement of transgenes designed to produce proteins for human therapy… 
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Until recently the only ways of making transgenic livestock were by pro-nuclear injection or, more controversially, by sperm mediated DNA transfer. Both techniques usually result in multiple copies
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