Production of functional chimaeric mouse/human antibody

  title={Production of functional chimaeric mouse/human antibody},
  author={Gabrielle L. Boulianne and Nobumichi Hozumi and Marc J. Shulman},
The availability of monoclonal antibodies has revived interest in immunotherapy. The ability to influence an individual's immune state by administering immunoglobulin of the appropriate specificity may provide a powerful approach to disease control and prevention. Compared with immunoglobulin from other species, human immunoglobulin (Ig) might be best for such therapeutic intervention; it might function better with the recipient's effector cells and should itself be less immunogenic. The… 

Production of Human Monoclonal Antibodies in SCID Mouse

A method to obtain HuMAbs with high antigen binding affinity by optimizing the human immune response to specific antigens in the Hu-PBL-SCID mouse model is described.

Design and engineering human forms of monoclonal antibodies

Different strategies for designing and engineering human antibodies and their derivatives are compared and summed up in this article.

Antibody Molecules, Genetic Engineering of

This article summarizes and compares different strategies for developing recombinant antibodies and their derivatives and examines their ability to trigger human immune effector mechanisms.

The diversity of antigen‐specific monoclonal antibodies from transgenic mice bearing human immunoglobulin gene miniloci

Although serum responses were relatively weak, monoclonal antibodies were readily obtained to all immunogens tested (a hapten, foreign proteins and human lymphoma cells) and suggests that the miniloci need not be particularly large.

Genetically engineered antibody molecules.

Preparation of genetically engineered monoclonal antibodies for human immunotherapy.

  • P. Parren
  • Biology
    Human antibodies and hybridomas
  • 1992
Recombinant DNA techniques to transplant the specificity of a rodent MAb into a human antibody can be used to study human Fc-dependent effector mechanisms in detail, which seems essential to optimize potential in vivo application.

The genetic engineering of monoclonal antibodies.

New Techniques for the Production of Therapeutic Recombinant Human Monoclonal Antibodies

Strategies in the development of therapeutic recombinant human antibodies are reviewed, which can be 'humanised' and tailored to the particular clinical application by antibody engineering.

Humanization of monoclonal antibodies.




Biological and clinical implications of lymphocyte hybridomas: tumor therapy with monoclonal antibodies.

Preliminary studies show that problems such as antigenic modulation, circulating free antigen, effector cell shortage, and host anti-mouse immunoglobulin response must be overcome.

A better cell line for making hybridomas secreting specific antibodies

The identification of such a cell line, Sp2/0-Ag14, is reported here the identification of a tumour cell fusion partner that makes no Ig but which can nevertheless be fused with spleen cells to obtain hybrids secreting only the specific antibody.

Switch from hapten-specific immunoglobulin M to immunoglobulin D secretion in a hybrid mouse cell line.

  • M. NeubergerK. Rajewsky
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1981
From a hybrid mouse cell line (B1-8), selection for a variant with lambda 1 chains on the surface resulted in the isolation of a line that had switched from mu to delta expression, which exists predominantly in the delta 2 lambda A2 form, does not contain J protein, is relatively stable in serum, and does not fix complement.

Transfer of a cloned immunoglobulin light-chain gene to mutant hybridoma cells restores specific antibody production

It is reported here that a cloned κ-chain gene is expressed in immunoglobulin-producing hybridoma cells and the product of the transferredκ- chain gene is capable of restoring specific antibody production to the transformed cells.

Biochemical genetics of the mouse IgM system.

  • M. ShulmanR. Hawley N. Hozumi
  • Biology
    Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire
  • 1984
Using a mouse hybridoma system, methods of isolating a variety of mutant cell lines in which immunoglobulin function or synthesis is defective are developed and a transfer system for the mu and kappa genes is described which will be useful in analyzing the structural basis of IgM function.

Mutations affecting the structure and function of immunoglobulin M

Using a hybridoma cell line which secretes hapten-specific immunoglobulin M (IgM), a variety of mutants which produce abnormal immunoglOBulin are isolated to provide a means of identifying the structural basis of IgM function and of studying the biochemistry of Igm synthesis and processing.

Expression of a VHCκ chimaeric protein in mouse myeloma cells

It is reported here that, when introduced into a mouse myeloma cell line, the chimaeric gene is expressed and a protein of the expected molecular weight is secreted into the medium.

Genetics, expression, and function of idiotypes.

It is seen that idiotypic interactions seem to be involved in the growth control of lymphocyte clones with defined receptor specificity and may thus be essential for the generation of a diverse system of interacting cells.