Production of functional bacteriorhodopsin by an Escherichia coli cell-free protein synthesis system supplemented with steroid detergent and lipid.

@article{Shimono2009ProductionOF,
  title={Production of functional bacteriorhodopsin by an Escherichia coli cell-free protein synthesis system supplemented with steroid detergent and lipid.},
  author={Kazumi Shimono and Mie Goto and Takashi Kikukawa and Seiji Miyauchi and Mikako Shirouzu and Naoki Kamo and Shigeyuki Yokoyama},
  journal={Protein science : a publication of the Protein Society},
  year={2009},
  volume={18 10},
  pages={2160-71}
}
Cell-free expression has become a highly promising tool for the efficient production of membrane proteins. In this study, we used a dialysis-based Escherichia coli cell-free system for the production of a membrane protein actively integrated into liposomes. The membrane protein was the light-driven proton pump bacteriorhodopsin, consisting of seven transmembrane alpha-helices. The cell-free expression system in the dialysis mode was supplemented with a combination of a detergent and a natural… CONTINUE READING
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