Production of fertile transgenic lentil (Lens culinaris Medik) plants using particle bombardment

@article{Gulati2007ProductionOF,
  title={Production of fertile transgenic lentil (Lens culinaris Medik) plants using particle bombardment},
  author={Anju Gulati and P. A. Schryer and Alan McHughen},
  journal={In Vitro Cellular & Developmental Biology - Plant},
  year={2007},
  volume={38},
  pages={316-324}
}
A reproducible system for gene transfer in lentil through particle bombardment is presented. Lentil cotyledonary nodes excised from germinated seedlings were bombarded with a plasmid containing a mutant acetolactate synthase gene (ALS) from tobacco conferring resistance to sulfonylurea herbicides. Putative transgenic shoots regenerated on Murashige and Skoog medium supplemented with 6-benzylaminopurine (BA) and chlorsulfuron (5 nM for first 4 wk followed by 2.5 nM for the remainder of the… CONTINUE READING