Production of extracellular fructans by Gluconobacter nephelii P1464

  title={Production of extracellular fructans by Gluconobacter nephelii P1464},
  author={P. Semjonovs and L. Shakirova and R. Treimane and K. Shvirksts and L. Auziņa and I. Cleenwerck and P. Zikmanis},
  journal={Letters in Applied Microbiology},
Bacterial extracellular fructans, known as levans, have potential applications in food, pharmaceutical and cosmetic industries and high fructan producing strains could contribute into the cost reduction and more extensive commercial usage of them. An acetic acid bacteria (AAB) isolate P1464 was obtained from the Microbial Strain Collection of Institute of Microbiology and Biotechnology, University of Latvia and identified as Gluconobacter nephelii by DNA‐DNA hybridization and the formation of… Expand
Biosynthesis and prebiotic activity of a linear levan from a new Paenibacillus isolate.
Microbiome and metabolome analysis showed that Plev intervention increased the abundance of beneficial bacteria and elevated the levels of short-chain fatty acids (SCFAs) in feces of mice, and could be considered a potential thickener and prebiotic supplement in food industry. Expand
Enhanced production and immunomodulatory activity of levan from the acetic acid bacterium, Tanticharoenia sakaeratensis.
This study reveals that T. sakaeratensis can be potentially employed as a new source of levan for industrial applications, and can promote nitric oxide production in RAW264, suggesting it has immunomodulatory effects. Expand
Size-Dependent Rheological Variability of Levan Produced by Gluconobacter Albidus TMW 2.1191
Levan is a fructan-type exopolysaccharide, which is produced by many microbes from sucrose via extracellular levansucrases. The hydrocolloid properties of levan depend on its molecular weight, whileExpand
Acetic acid bacteria encode two levansucrase types of different ecological relationship.
Two LS types encoded by AAB are proposed as determinants for habitat selection, which emerged from vertical (T1) and horizontal (T2) lines of evolution and differ in their genetic organization, structural features and secretion mechanism, as well as their occurrence in proteobacteria. Expand
Production of 1,3-Dihydroxyacetone by Gluconobacter nephelii in Upflow Aerated Bioreactors with Agro-industrial Wastes as External Nitrogen Source
Cultivation of recently identified Gluconobacter nephelii to produce 1,3dihydroxyacetone (DHA) was conducted using the growth medium containing the external nitrogen source from agro-industrialExpand
Insights into the pH-dependent, extracellular sucrose utilization and concomitant levan formation by Gluconobacter albidus TMW 2.1191
Findings indicate the physiological adaptation of G. albidus TMW 2.1191 to efficient colonisation of sucrose-rich habitats via released levansucrases despite changing extracellular pH conditions in course of acid formation. Expand
Size-Dependent Variability in Flow and Viscoelastic Behavior of Levan Produced by Gluconobacter albidus TMW 2.1191
The study reveals that the rheological properties of levan are determined by its size and polydispersity, rather than by the amount of leVan used or the structural composition. Expand
Production of biodegradable microbial polymers from whey
Current research in industrial microbiology and biotechnology focuses on the production of biodegradable microbial polymers as an environmentally friendly alternative to still dominant fossil-basedExpand
Kombucha Tea Fermentation: A Review
Abstract Kombucha tea is made by aerobically fermenting a sweetened tea infusion with a kombucha culture, a symbiotic culture of bacteria and yeasts. The resulting beverage is usually non-alcoholic,Expand


Structural analysis of fructans produced by acetic acid bacteria reveals a relation to hydrocolloid function.
The data suggest that the increasing molecular weight of a levan particle enforces intramolecular interactions to reach the structural compactness of a microgel with hydrocolloid properties. Expand
Microbial levan from Pseudomonas fluorescens: Characterization and medium optimization for enhanced production
Levan, a polyfructan which consists of D-fructofuranosyl residues linked predominantly by β-(2,6) linkage as a core chain with some β-(2,1) branch chains have potential applications in theExpand
Sucrose utilization by Zymomonas mobilis: Levan production optimization using submerged fermentation
Levan is an extracellular polysaccharide (EPS), constituted by linked fructose units β (2,6), obtained by transfructosilation reaction during fermentation of microorganisms in a sucrose rich cultureExpand
The Genus Gluconobacter Oxydans: Comprehensive Overview of Biochemistry and Biotechnological Applications
This review aims to give an overview of the myriad of applications for Gluconobacter, with a special focus on some recent developments. Expand
Kinetic behaviour and specificity of β-fructosidases in the hydrolysis of plant and microbial fructans
Abstract Fructosidases, in particular exo-β-fructosidases, may act on fructans such as inulins and levans of plant and bacterial origin to produce fructose. In this paper, the kinetic properties of aExpand
Prebiotic Potential of Agave angustifolia Haw Fructans with Different Degrees of Polymerization
The objective of this work was to assess the prebiotic potential of three Agave angustifolia fructan fractions (AFF) with different degrees of polymerization, which assessed in vitro through fermentation by Bifidobacterium and Lactobacillus strains. Expand
Microbial levan.
  • Y. W. Han
  • Medicine
  • Advances in applied microbiology
  • 1990
Levans are natural polymers of the sugar fructose found in many plants and microbial products that have potential industrial applications as thickeners and encapsulating agents and could provide additional, valuable products from sugarcane juice. Expand
Influence of Nutritional Factors on the Nature, Yield, and Composition of Exopolysaccharides Produced by Gluconacetobacter xylinus I-2281
Although the activity of key enzymes showed large differences to be a function of the carbon source, the ratios of their activities remained similar from one carbon source to another and corresponded to the ratio of precursor needs as deduced from the gluconacetan composition. Expand
Antioxidant and anti-inflammatory levan produced from Acetobacter xylinum NCIM2526 and its statistical optimization.
This study has demonstrated the microbial production of therapeutically potential levan by batch fermentation process in sucrose rich medium using Acetobacter xylinum NCIM 2526 and focused on the optimization of levan production using one factor at a time approach followed by a statistical method, central composite design (CCD) with selected variables. Expand
Production of Biopolymer Levan by Bacillus subtilis using Non-Ionic Surfactants
Levan, an exoploysaccharide, was produced by Bacillus subtilis and its yield was characterized as a function of saccharose concentration (10%) and fermentation time (96h).Under these conditions ,Expand