Production of double haploids in oat (Avena sativa L.) by pollination with maize (Zea mays L.)

@article{Marciska2013ProductionOD,
  title={Production of double haploids in oat (Avena sativa L.) by pollination with maize (Zea mays L.)},
  author={Izabela Marcińska and Agata Nowakowska and Edyta Skrzypek and Ilona Mieczysława Czyczyło-Mysza},
  journal={Central European Journal of Biology},
  year={2013},
  volume={8},
  pages={306-313}
}
The aim of the study was to optimize the method of oat haploid production by pollination with maize. Seventeen oat genotypes were used in the experiment. Various factors influencing the growth and development of ovaries and embryo production were investigated: genotype, time of pollination, growth regulators and time of their application. Emasculated before anthesis, oat florets were pollinated with maize pollen after 0, 1 or 2 days. Next, one of two auxins analogues (2,4-D or dicamba) were… 

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