Production of deuterated switchgrass by hydroponic cultivation

  title={Production of deuterated switchgrass by hydroponic cultivation},
  author={Barbara R. Evans and Garima Bali and Marcus B. Foston and Arthur J. Ragauskas and Hugh M. O'Neill and Riddhi Shah and J. Mcgaughey and David T. Reeves and Caroline Rempe and Brian H. Davison},
AbstractMain conclusionThe bioenergy crop switchgrass was grown hydroponically from tiller cuttings in 50 % D2O to obtain biomass with 34 % deuterium substitution and physicochemical properties similar to those of H2O-grown switchgrass controls. Deuterium enrichment of biological materials can potentially enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls. Two key advances have… 
Production of deuterated biomass by cultivation of Lemna minor (duckweed) in D2O
The production of biomass with 50–60% deuterium incorporation by long-term photoheterotrophic growth of common duckweed Lemna minor in 50% D2O with 0.5% glucose is reported.
Effect of in Vivo Deuteration on Structure of Switchgrass Lignin
Biomass deuteration is an effective engineering method that can be used to provide key insights into understanding of biomass recalcitrance and the complex biomass conversion process. In this study,
Allelopathic effects of exogenous phenylalanine: a comparison of four monocot species
Main conclusionExogenous phenylalanine stunted annual ryegrass but not switchgrass or winter grain rye, with deuterium incorporation up to 3% from phenyalanine-d8. Toxicity to duckweed varied with
Deuteration Effects on Switchgrass Structure and Metabolism: Lignin Deposition Changes in Cell Walls of Deuterated Switchgrass
Project Goals: Lignocellulosic biomass comprises the vast majority of biomass on Earth and has the potential to play a major role in generation of renewable biofuels if costeffective conversion can
Ultrastructure and Enzymatic Hydrolysis of Deuterated Switchgrass
Transmission electron microscopy showed differences in lignin distribution and packing of fibers in the cell walls that apparently increased surface area of cellulose in deuterated switchgrass, increasing cellulose accessibility and lowering its recalcitrance.
Hemicellulose characterization of deuterated switchgrass.
Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.
Development of approaches for deuterium incorporation in plants.
Cultivation in 50% D2O of annual ryegrass and switchgrass following establishment of roots by growth in H2O produces samples with normal morphology and 30-40% deuterium incorporation in the biomass, and changes in infrared (IR) absorption observed for H to D substitution in hydroxyl and alkyl groups provide rapid initial evaluation of incorporation.
Funaria hygrometrica Hedw. elevated tolerance to D2O: its use for the production of highly deuterated metabolites
The ability to tolerate high concentrations of D2O together with the development of a rich molecular toolbox makes F. hygrometrica an ideal system for the production of valuable deuterated metabolites.
Effects of Media Composition and Auxins on Adventitious Rooting of Bienertia sinuspersici Cuttings
Overall, the established procedure provides a simple and cost-effective means for the rapid propagation of the single-cell C4 species B. sinuspersici.
The preparation and characterization of chemically deuterium incorporated cotton fibers
Preparation of deuterium incorporated cellulose is a vital tool to investigate cellulose internal structure and to expand the application fields of cellulose materials. In this study, cellulosic


Effect of D2O on growth properties and chemical structure of annual ryegrass (Lolium multiflorum).
A method for optimized production of deuterated annual ryegrass (Lolium multiflorum) was developed by growing the plants in 50% D2O in perfused hydroponic chambers and deuterium incorporation was found in the annual rye.
Adaptation and growth response of Arabidopsis thaliana to deuterium
SummaryArabidopsis seeds were sown aseptically on mineral media containing between 0 and 90% of heavy water (D2O). Initially, a D2O level of over 50% was lethal for the plants. However, after culture
Molecular breeding of switchgrass for use as a biofuel crop.
  • J. Bouton
  • Biology, Medicine
    Current opinion in genetics & development
  • 2007
Switchgrass (Panicum virgatum L.) is projected to become one of the main herbaceous, biofuel crops in United States and will include production of high yielding hybrids and the use of genomic and transgenic biotechnologies to enhance both productivity and chemical composition.
Development of In Vitro Systems for Switchgrass (Panicum virgatum) - Final Report for 1992 to 2002
Alamo (lowland type) was much more amenable to in vitro culture, and plants could be regenerated much more easily than from Cave-in-Rock (upland type), and the lowland cultivars Alamo and Kanlow were much easier to regenerate than upland cultivars, such as Trailblazer, Blackwell, and Cave- in-Rock.
SANS study of cellulose extracted from switchgrass.
Small-angle neutron scattering was employed to probe the multi-scale structure of cellulosic materials and found the high-Q structural feature (Q > 0.07 Å(-1)) can be assigned to cellulose fibrils based on a comparison of cellulose purified by solvent extraction of native and dilute acid pretreated switchgrass and a commercial preparation of microcrystalline cellulose.
Changes in lignocellulosic supramolecular and ultrastructure during dilute acid pretreatment of Populus and switchgrass
Abstract Dilute acid pretreatment (DAP) is commonly employed prior to enzymatic deconstruction of cellulose to increase overall sugar and subsequent ethanol yields from downstream bioconversion
Switchgrass as an energy crop for biofuel production: A review of its ligno-cellulosic chemical properties
Over the past two decades, the use of biomass as a resource for biofuels and bioenergy has garnered much interest. The reduction in green house gas emissions of renewable fuels as compared to
Biomass Characterization: Recent Progress in Understanding Biomass Recalcitrance
Biological or enzymatic hydrolysis offers the potential for low-cost, high-yield, and selective production of targeted chemicals and value-added co-products at milder operating conditions than thermochemical processes such as gasification or pyrolysis.
Germination and Seedling Growth of Winter Rye in Deuterium Oxide
WHILE investigating environmental stress factors in seed germination and seedling growth, winter rye (Secale cereale) was found to be exceptionally resistant to a wide variety of ordinarily harmful
The Evolution of Switchgrass as an Energy Crop
This chapter discusses the prehistoric origins of switchgrass, its mid-twentieth century adoption as a crop, and late-twentieth century efforts to develop it into an energy crop. The species probably