Production of dengue 2 envelope domain III in plant using TMV-based vector system.

  title={Production of dengue 2 envelope domain III in plant using TMV-based vector system.},
  author={Wanida Saejung and Kazuhito Fujiyama and Tomohiko Takasaki and Mikako Ito and Koichi Hori and Prida Malasit and Yuichiro Watanabe and Ichiro Kurane and Tatsuji Seki},
  volume={25 36},
The envelope protein of dengue virus is the major protein involved in host cell receptor binding for viral entry and induction of immunity. A gene fragment encoding domain III of the dengue 2 envelope protein (D2EIII, amino acids 298-400) was successfully expressed in Nicotinana benthamiana plant using a tobacco mosaic virus (TMV)-based transient expression system. The N-terminal 5' untranslated region-omega sequence located upstream of D2EIII increased protein production in infected plant… CONTINUE READING


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