Production of clones of homozygous diploid zebra fish (Brachydanio rerio)

  title={Production of clones of homozygous diploid zebra fish (Brachydanio rerio)},
  author={George Streisinger and Charline Walker and Nancy A. Dower and Donna Knauber and Fred Singer},
Homozygous diploid zebra fish have been produced on a large scale by the application of simple physical treatments. Clones of homozygous fish have been produced from individual homozygotes. These clones and associated genetic methods mil facilitate genetic analyses of this vertebrate. 

Production of cloned fish by chromosome manipulation and confirmation by DNA markers

The production of cloned fish by chromosome manipulation is outlined, which shows clear trends in the use of clones as commercial strains in aquaculture.

Production and confirmation of clones using gynogenesis in Japanese flounder

Two homozygous clones of Japanese flounder were produced by the gynogenesis that suppresses the cleavage of the eggs in the mitosis and then blocks the extrusion of the second polar body in the meiosis, demonstrating the successful development of cloning.

Genetic engineering in fish.

Several genetic manipulation techniques are being actively investigated in a number of fish species, and induced triploidy and induction of homozygous diploids do not appear to be possible in mammals.

Genetic techniques for control of sexuality in fish farming

  • C. Purdom
  • Biology
    Fish Physiology and Biochemistry
  • 2005
Techniques include the development of a range of spawning times, the control of sex-ratio and the elimination of sexual maturity by induced polyploidy.

Residual paternal inheritance in gynogenetic rainbow trout: implications for gene transfer

The results indicate that paternal chromosome fragments are genetically active in gynogenetic offspring, but that these fragments may be lost during mitotic cell division, producing mosaic fish.

Reconstitution of Genetic Strains of Salmonids Using Biotechnical Approaches

Genetic conservation of the existing salmonid stocks is an important goal in itself and as a component of programs designed to insure viable and sustainable fisheries under changing environmental

Production of heterozygous and homozygous clones in Nile tilapia

Production of heterozygous and homozygous clones of Nile tilapia (Oreochromis niloticus L.) was successfully carried out and a model for the large scale production of similar clones is presented.

Artifical gynogenesis and mapping of gene-centromere distances in the paradise fish, Macropodus opercularis

SummaryAn efficient method has been developed for the production of gynogenetic paradise fish in order to obtain genetically homogeneous strains in a relatively short period. Diploidy of the

Production of Cloned Amago Salmon Oncorhynchus rhodurus* 3-5

The mitotic-G2N method is not influenced by gene-centromere recombination for induction of complete homozygous progeny and is indispensable for inducing the clonal lines.



Homozygous mouse embryos produced by microsurgery.

Homozygous mouse blastocysts have been produced following microsurgical removal of one pronucleus from the fertilized egg, diploidization in cytochalasin B, and culture in vitro, which should greatly shorten the time required to obtain homozygous strains of mice.

The genetics of Caenorhabditis elegans.

Estimates of the induced mutation frequency of both the visible mutants and X chromosome lethals suggests that, just as in Drosophila, the genetic units in C. elegans are large.

Effect of temperature on early embryological development of the zebra fish, Brachydanio rerio†

The early development of the zebra fish embryo, Brachydanio rerio (Hamilton) was studied under thermostatically controlled temperatures ranging from 13° to 35°C. Regular and successive cleavages and

Induced polyploidy in plaice (Pleuronectes platessa) and its hybrid with the flounder (Platichthys flesus)

Induced polyploidy in plaice ( Pleuronectes platessa ) and its hybrid with the flounder ( Platichthys flesus )

Microsurgically produced homozygous-diploid uniparental mice.

  • P. HoppeK. Illmensee
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1977
Six of the seven homozygous-diploid (isogeneic) females have proved to be fertile and have given birth to progeny corresponding only to the pronuclear genotype of the mother, depending on whether the female or male pronucleus had been retained in the egg.

Heat-Induced Triploidy in the Newt, Triturus Viridescens.

  • G. FankhauserR. C. Watson
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1942
Heat and cold have both been widely used to induce polyploidy in plants."' 2, 3 4 Depending on whether the treatment is applied during meiosis or during the early divisions of the zygote, triploid or

Rapid Chromosome Preparations from Solid Tissues of Fishes

A technique is described for obtaining well-spread metaphases from solid tissues of fishes without the use of methodologies that rely on tissue grinders, centrifuges, digestive enzymes, or tissue


The following topics are discussed: susceptibility of rainbow trout (Salmo gairdneri) to aflatoxins and other mycotoxins; the Oregon test diet; carcinogenic effects of cyclopropenoid fatty acids;

Mapping Centromeres in the Axolotl.

In organisms from which the products of meiosis are recovered singly it is not generally possible to determine whether the first division is equational or reductional; when half of or all the meiotic products are recoverable in a known order, equational and reductional separation of a marker can be scored and its location in relation to the centromere can be determined.