Production of cloned pigs from in vitro systems

@article{Betthauser2000ProductionOC,
  title={Production of cloned pigs from in vitro systems},
  author={Jeff M Betthauser and Erik Jordahl Forsberg and Monica L Augenstein and Lynette A Childs and Kenneth J. Eilertsen and Joellyn M Enos and Todd M Forsythe and Paul J. Golueke and Gail F Jurgella and Richard W Koppang and Tiffany L Lesmeister and Kelly S Mallon and Greg D Mell and Pavla M Misica and Marvin M. Pace and Martha Pfister-Genskow and Nikolai S Strelchenko and Gary R. Voelker and Steven R Watt and Simon Giles Thompson and M. J. D. Bishop},
  journal={Nature Biotechnology},
  year={2000},
  volume={18},
  pages={1055-1059}
}
Here we describe a procedure for cloning pigs by the use of in vitro culture systems. Four healthy male piglets from two litters were born following nuclear transfer of cultured somatic cells and subsequent embryo transfer. The initiation of five additional pregnancies demonstrates the reproducibility of this procedure. Its important features include extended in vitro culture of fetal cells preceding nuclear transfer, as well as in vitro maturation and activation of oocytes and in vitro embryo… CONTINUE READING
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