Production of cell-associated polysaccharides of Rhizobium fredii USDA205 is modulated by apigenin and host root extract.

@article{Reuhs1994ProductionOC,
  title={Production of cell-associated polysaccharides of Rhizobium fredii USDA205 is modulated by apigenin and host root extract.},
  author={Bradley L. Reuhs and J S Kim and A A Badgett and Russell W Carlson},
  journal={Molecular plant-microbe interactions : MPMI},
  year={1994},
  volume={7 2},
  pages={240-7}
}
Rhizobium fredii USDA205 cells were cultured in the presence of 4',5,7-trihydroxyflavone (apigenin), a compound that has been shown to induce the nod genes and other symbiosis-related genes in R. fredii. The cell-associated polysaccharides were then extracted with hot phenol/water, separated by repetitive gel filtration chromatography, and analyzed by polyacrylamide gel electrophoresis, nuclear magnetic resonance spectrometry, high-performance anion-exchange chromatography, and gas… CONTINUE READING