Production of brefeldin-A

@article{McCloud2005ProductionOB,
  title={Production of brefeldin-A},
  author={Thomas G. McCloud and Michael P. Burns and Fathy Majadly and Gary M. Muschik and Debi Miller and Karyol Poole and John M Roach and John T. Ross and William B. Lebherz},
  journal={Journal of Industrial Microbiology},
  year={2005},
  volume={15},
  pages={5-9}
}
Fermentation conditions are described for the production of the antitumor antibiotic 7-(S)-brefeldin-A (brefeldin-A) in liquid culture byEupenicillium brefeldianum, (B. Dodge) Stolk and Scott, ATCC 58665. An analytical hplc method was developed which allowed rapid quantitation of the compound during fermentation. A kilogram of brefeldin-A was isolated from a fermentation at the 6800-liter scale. 
View on Springer
zenodo.org
10 Citations
Background Citations
4
Methods Citations
1

10 Citations

Citation Type

Citation Type

Development of macrolide lactone antibiotic brefeldin A fermentation process with Eupenicillium brefeldianum ZJB082702.
trans-Hydrogenation: Application to a Concise and Scalable Synthesis of Brefeldin A**
TLDR
This work hinges upon the first implementation of ruthenium-catalyzed trans-hydrogenation in natural products total synthesis and outperforms the classical Birch-type reduction that could not be applied at such a late stage.
The synthesis of 3-azabicyclo[4.3.0]nonane scaffolds from brefeldin A
Endophytic Fungi as a Source of Antiviral Compounds – A Review
TLDR
It is demonstrated that endophytic fungi are a rich source of secondary metabolites that combat pathologies caused by viruses, and practical and biotechnological screening tools for the research of these metabolites will provide promising drugs to combat these infections.
High Throughput Extraction of Plant, Marine and Fungal Specimens for Preservation of Biologically Active Molecules
TLDR
Details of high throughput extract production for preservation of biologically active molecules are presented, which yields both an organic solvent soluble and a water soluble material for inclusion into the screening library.
Fungal Polyketide Synthase Product Chain-Length Control by Partnering Thiohydrolase
TLDR
Contrary to what is expected from the structure of BFA, Bref-PKS is found to be a nonaketide synthase in the absence of an associated thiohydrolase BreF-TH, and highlights the importance of including tailoring enzyme activities in predicting fungal HRPKS functions and their products.
cis-Golgi proteins accumulate near the ER exit sites and act as the scaffold for Golgi regeneration after brefeldin A treatment in tobacco BY-2 cells
Particular cis-Golgi proteins accumulate in novel punctate structures close to ERES by BFA treatment in tobacco BY-2 cells. These structures reassemble first to form cis-Golgi after BFA removal, and…
Dynamic behavior of the trans-golgi network in root tissues of Arabidopsis revealed by super-resolution live imaging.
TLDR
It is found that the dynamic features of the TGN in plant cells differ from those of animal and yeast cells, and the abundance of the GI-TGNs differs between observed tissues.
Cell wall accumulation of fluorescent proteins derived from a trans-Golgi cisternal membrane marker and paramural bodies in interdigitated Arabidopsis leaf epidermal cells
In most dicotyledonous plants, leaf epidermal pavement cells develop jigsaw puzzle-like shapes during cell expansion. The rapid growth and complicated cell shape of pavement cells is suggested to be…
Discovery of Potential Anticancer Agents from Aquatic Cyanobacteria, Filamentous Fungi, and Tropical Plants

References

SHOWING 1-10 OF 59 REFERENCES
7-epi-Brefeldin A, a co-metabolite of brefeldin A in Curvularia lunata
The macrolide antibiotic brefeldin A and its 7-epimer have been isolated from cultures of Curvularia lunata. The structure of 7-epi-brefeldin A was determined by spectrometric and chemical means.
Biosynthesis of brefeldin A. Introduction of oxygen at the C-7 position.
TLDR
The biosynthetic introduction of oxygen in position 7 of brefeldin A, a structurally unique macrolide with an alicyclic ring, was studied and clearly indicates that the oxygen does not contribute to the cyclopentane ring formation but is introduced during the last step of brefieldin A biosynthesis.
The biosynthesis of brefeldin A
In contrast to the observation that palmitate acts as a specific precursor of brefeldin A (1) in P. cyaneum, feeding [16-14C]palmitate to P. lilacinum gave uniformly labelled brefeldin A.
Comparative biochemistry of fatty acid and macrolide antibiotic (brefeldin a). Formation in penicillium brefeldianum
Macrolide biosynthesis: stereochemistry of the hydroxylation of brefeldin C.
TLDR
Using stereospecifically 2H-labeled forms of 2, the hydroxylation of 2 to 1 is shown to involve stereosPEcific removal of the 7 pro-S hydrogen from 2.
Eupenifeldin, a novel cytotoxic bistropolone from Eupenicillium brefeldianum.
TLDR
Eupenifeldin was isolated from cultures of Eupenicillium brefeldianum by extraction and crystallization and its complete structure was established by single-crystal X-ray analysis.
Über die Isolierung neuer Stoffwechselprodukte aus Penicillium brefeldianum DODGE
Two new mold metabolites, Brefeldin A (C16H24O4) and Brefeldin B (C15H2205), have been isolated from the fermentation broth of a strain of Pefiicillium brefeldiawm DODGE. Their chemical, physical and…
Decumbin, a New Compound from a Species of Penicillium
TLDR
It is concluded that an organism isolated during a microbiological study of corn spoiled in storage produced an aroma reminiscent of exaltone and crystalline bodies in artificial culture that is a previously unreported dihydroxy, α-β unsaturated lactone (C16H24O4).
The distribution of secretory products in plant cells is affected by brefeldin A
TLDR
The preliminary results described here demonstrate that the combination of immunocytochemical techniques with drug induced perturbation of secretory pathways will be invaluable in enhancing the understanding of the pathways of intracellular trafficking in plants.
Die Konstitution von Brefeldin A
The constitution of Brefeldin A, a mold metabolite from Penicillium brefeldianum DODGE, has been elucidated as 1 by chemical degradation.
...
...