Production of bacteriocin E50-52 by small ubiquitin-related modifier fusion in Escherichia coli.

@article{Wang2013ProductionOB,
  title={Production of bacteriocin E50-52 by small ubiquitin-related modifier fusion in Escherichia coli.},
  author={Qing Wang and Wenjuan Fu and Qingshan Ma and Zhanqiao Yu and Rijun Zhang},
  journal={Polish journal of microbiology},
  year={2013},
  volume={62 4},
  pages={
          345-50
        }
}
Bacteriocin E50-52, a class IIa bacteriocin with a wide antibacterial spectrum, and has a huge potential to be a substitute for convention. antibiotics. In this research, the bacteriocin E50-52 gene was cloned into the expression vector pET SUMO (small ubiquitin-related modifier) and introduced into Escherichia coli BL21 (DE3). The recombinant fusion protein SUMO-bacteriocin E50-52 expressed in a soluble form was purified to a purity of more than 90% by Ni-NTA sepharose column and 117 mg fusion… CONTINUE READING