Production of authentic SARS-CoV M(pro) with enhanced activity: application as a novel tag-cleavage endopeptidase for protein overproduction.

@article{Xue2007ProductionOA,
  title={Production of authentic SARS-CoV M(pro) with enhanced activity: application as a novel tag-cleavage endopeptidase for protein overproduction.},
  author={Xiaoyu Xue and Haitao Yang and Wei Shen and Qi Zhao and Jun Li and Kailin Yang and Cheng Chen and Yinghua Jin and Mark Bartlam and Zihe Rao},
  journal={Journal of molecular biology},
  year={2007},
  volume={366 3},
  pages={965-75}
}
The viral proteases have proven to be the most selective and useful for removing the fusion tags in fusion protein expression systems. As a key enzyme in the viral life-cycle, the main protease (M(pro)) is most attractive for drug design targeting the SARS coronavirus (SARS-CoV), the etiological agent responsible for the outbreak of severe acute respiratory syndrome (SARS) in 2003. In this study, SARS-CoV M(pro) was used to specifically remove the GST tag in a new fusion protein expression… CONTINUE READING
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