Production of antigen-specific human monoclonal antibodies from in vitro-primed human splenocytes.

@article{Boerner1991ProductionOA,
  title={Production of antigen-specific human monoclonal antibodies from in vitro-primed human splenocytes.},
  author={Paula Boerner and Ronald Lafond and Weizhen Lu and Peter Brams and Ivor Royston},
  journal={Journal of immunology},
  year={1991},
  volume={147 1},
  pages={86-95}
}
We have developed culture conditions for human lymphocytes that support primary in vitro immune responses to protein Ag of either human or nonhuman origin. We now show that these primed B cells can be efficiently immortalized by fusion with a heterohybrid fusion partner to generate human, Ag-specific IgM or IgG antibody-producing heterohybridomas at a rate of 17 to 50 hybrids/10(6) lymphocytes fused. Approximately 50% of the Ig-secreting clones were stable with respect to Ig secretion. Levels… CONTINUE READING