Production of anti-carbohydrate antibodies by phage display technologies: potential impairment of cell growth as a result of endogenous expression.

@article{Yuasa2010ProductionOA,
  title={Production of anti-carbohydrate antibodies by phage display technologies: potential impairment of cell growth as a result of endogenous expression.},
  author={Noriyuki Yuasa and Wei Zhang and Tomohiro Goto and Hiroyuki Sakaue and Ayano Matsumoto-Takasaki and Miyo Kimura and Hiroya Ohshima and Yasunobu Tsuchida and Tomoyuki Koizumi and Keiko Sakai and Takumi Kojima and Kazuo Yamamoto and Munehiro Nakata and Yoko Fujita-Yamaguchi},
  journal={The Journal of biological chemistry},
  year={2010},
  volume={285 40},
  pages={30587-97}
}
Anti-mannotriose (Man3) antibodies were previously isolated from a Keio phage library displaying human single chain variable fragments (scFvs) using a neoglycolipid, Man3- dipalmitoylphosphatidylethanolamine. Of three genes constructed, the 5A3 clone was expressed in mouse myeloma NS0 cells as a conjugate with human IgG(1) Fc (scFv-Fc) and characterized (Sakai, K., Shimizu, Y., Chiba, T., Matsumoto-Takasaki, A., Kusada, Y., Zhang, W., Nakata, M., Kojima, N., Toma, K., Takayanagi, A., Shimizu, N… CONTINUE READING