Production of androgenetic zebrafish (Danio rerio).

@article{CorleySmith1996ProductionOA,
  title={Production of androgenetic zebrafish (Danio rerio).},
  author={Graham E. Corley-Smith and Chinten James Lim and Bruce Peter Brandhorst},
  journal={Genetics},
  year={1996},
  volume={142 4},
  pages={1265-76}
}
To help investigate the evolutionary origin of the imprinting (parent-of-origin mono-allelic expression) of paternal genes observed in mammals, we constructed haploid and diploid androgenetic zebrafish (Danio rerio). Haploid androgenotes were produced by fertilizing eggs that had been X-ray irradiated to eliminate the maternal genome. Subsequent inhibition of the first mitotic division of haploid androgenotes by heat shock produced diploid androgenotes. The lack of inheritance of maternal… CONTINUE READING