Production of analytical quantities of recombinant proteins in Chinese hamster ovary cells using sodium butyrate to elevate gene expression.

@article{Palermo1991ProductionOA,
  title={Production of analytical quantities of recombinant proteins in Chinese hamster ovary cells using sodium butyrate to elevate gene expression.},
  author={D P Palermo and M E Degraaf and Keith R. Marotti and Elisabeth Rehberg and L E Post},
  journal={Journal of biotechnology},
  year={1991},
  volume={19 1},
  pages={35-47}
}
Sodium butyrate was used to enhance expression levels and thereby facilitate the generation of analytical quantities of nine different tissue plasminogen activator (tPA) analogues expressed under the control of the cytomegalovirus immediate early (CMV IE) promoter by the Chinese hamster ovary (CHO) mammalian expression system. Production involved growth in… CONTINUE READING