Production of active pigment epithelium-derived factor inE. coli

@article{Zhang2005ProductionOA,
  title={Production of active pigment epithelium-derived factor inE. coli},
  author={Tao Zhang and Ming Guan and Yuan Lu},
  journal={Biotechnology Letters},
  year={2005},
  volume={27},
  pages={403-407}
}
Human pigment epithelium-derived factor (PEDF), a neurotrophic factor, is the most potent natural inhibitor of angiogenesis. To produce the active PEDF, the gene coding for the human PEDF protein was expressed in E. coli. The rPEDF protein was expressed at 457 mg l−1 as a soluble protein. The yield of purified GST fusion protein was 14 mg ll−1. Purified rPEDF inhibited tube formation in endothelial cells. 

From This Paper

Figures, tables, results, connections, and topics extracted from this paper.
3 Extracted Citations
8 Extracted References
Similar Papers

Referenced Papers

Publications referenced by this paper.
Showing 1-8 of 8 references

Similar Papers

Loading similar papers…