Production of abnormal proteins in E. coli stimulates transcription of ion and other heat shock genes

@article{Goff1985ProductionOA,
  title={Production of abnormal proteins in E. coli stimulates transcription of ion and other heat shock genes},
  author={Stephen A. Goff and Alfred L Goldberg},
  journal={Cell},
  year={1985},
  volume={41},
  pages={587-595}
}
The product of the lon gene in Escherichia coli, protease La, plays an important role in the degradation of abnormal proteins. To determine whether the presence of abnormal proteins stimulates expression of this gene, we examined its transcription using a lon-lacZ operon fusion. After the cells synthesized large amounts of aberrant polypeptides (e.g. following incorporation of the arginine analog, canavanine, or production of incomplete proteins with puromycin, or induction of translational… CONTINUE READING

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