Production of a transgenic pig expressing human albumin and enhanced green fluorescent protein.

@article{Naruse2005ProductionOA,
  title={Production of a transgenic pig expressing human albumin and enhanced green fluorescent protein.},
  author={Katsutoshi Naruse and Hiroshi Ishikawa and Hiro-omi Kawano and Hideto Ueda and Mayuko Kurome and K{\^o}ji Miyazaki and Maiko Endo and Tohru Sawasaki and Hiroshi Nagashima and Masatoshi Makuuchi},
  journal={The Journal of reproduction and development},
  year={2005},
  volume={51 4},
  pages={539-46}
}
We introduced a fusion gene of human albumin and enhanced green fluorescent protein (EGFP) into porcine oocytes using the sperm vector method, and produced a piglet that showed clear expression of GFP in the hooves and skin. PCR and Southern blotting analysis of genomic DNA extracted from the piglet's tissues, including the liver, showed that the tissues carried the transgene. RT-PCR analysis demonstrated that both the human albumin and EGFP genes were expressed in the tissues. The fact that… CONTINUE READING