Production of a non-triple helical collagen alpha chain in transgenic silkworms and its evaluation as a gelatin substitute for cell culture.

@article{Adachi2010ProductionOA,
  title={Production of a non-triple helical collagen alpha chain in transgenic silkworms and its evaluation as a gelatin substitute for cell culture.},
  author={Takahiro Adachi and Xiaobiao Wang and Tomoko Murata and Masanobu Obara and Hidenori Akutsu and Masakazu Machida and Akirihiro Umezawa and Masahiro Tomita},
  journal={Biotechnology and bioengineering},
  year={2010},
  volume={106 6},
  pages={860-70}
}
We generated transgenic silkworms that synthesized human type I collagen alpha1 chain [alpha1(I) chain] in the middle silk glands and secreted it into cocoons. The initial content of the recombinant alpha1(I) chain in the cocoons of the transgenic silkworms was 0.8%. The IE1 gene, a trans-activator from the baculovirus, was introduced into the transgenic silkworm to increase the content of the chain. We also generated silkworms homozygous for the transgenes. These manipulations increased the… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 15 extracted citations

Similar Papers

Loading similar papers…