Production of a new antiserum to arthropod molting hormone and comparison with two other antisera.

@article{Soumoff1981ProductionOA,
  title={Production of a new antiserum to arthropod molting hormone and comparison with two other antisera.},
  author={Cynthia Soumoff and Denis H.S. Horn and John D. O'connor},
  journal={Journal of steroid biochemistry},
  year={1981},
  volume={14 5},
  pages={
          429-35
        }
}
Abstract β-ecdysone 2-hemisuccinate was conjugated to thyroglobulin yielding 166 haptens per molecule of protein. The antiserum raised against this conjugate had a strong binding affinity for β-ecdysone and displayed reduced affinity for β-ecdysone analogs with altered hydroxyl functions along the sidechain. One exception was α-ecdysone which competed more effectively than β-ecdysone for antibody binding sites. Antiserum sensitivity toward these two hormones varied over a fifteen week interval… 
Radioimmunoassay and the characterization of crustacean ecdysteroids
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The sensitivity of the RIA has permitted the monitoring of the rate of secretion of ecdysteroids by molting glands exposed to various in vitro conditions, and this latter approach will be of great utility in determining the nature and effect of molt regulators (inhibitors) present in other tissues.
Ecdysteroid titre and metabolism to novel apolar derivatives in adult female Boophilus microplus (Ixodidae)
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References

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Abstract The 22-hemisuccinate ester of α-ecdysone was conjugated to bovine thyroglobulin and used to raise an antiserum which has a greater affinity for α-ecdysone and an overall higher sensitivity
A radioimmunoassay for arthropod moulting hormones, introducing a novel method of immunogen coupling.
TLDR
The antiserum has a greater affinity for ecdysone and 22-isoecdysone than for all other ecdysteroids and steroids tested.
The qualitative and quantitative determinations of ecdysones in tissues of the crab, Pachygrapsus crassipes, following molt induction.
TLDR
From in vitro experiments, it is demonstrated that crab tissues have the ability to convert radiolabeled α- to β-ecdysone and the testis appears to have the greatest capacity for this conversion.
In vitro secretion and hydroxylation of α-ecdysone as a function of the crustacean molt cycle
TLDR
The ability to hydroxylate the Y-organ secretory product to the predominant circulating form of the molting hormone is concentrated in the mitochondria-enriched fraction of the testes of the crabs, Pachygrapsus crassipes and Cancer antennarius.
Metabolism of some possible ecdysone precursors in Calliphora stygia.
A study has been made, using Calliphora stygia at the time of puparium formation, of the incorporation of a number of labelled sterols into β-ecdysone. [1α-3H]-Cholesterol and [4-14C]-cholesterol are
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  • Biology, Medicine
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TLDR
This review article is an attempt to summarize recent developments in knowledge regarding the chemistry, synthesis and metabolism of the arthropod molting hormones, but will in the main exclude consideration of biological properties which have been frequently the object of excellent reviews.
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The number and variety of known compounrjs between proteins and small molecules are increasing rapidly and make a fascinating story. For instance, there are the compounds of iron, which is carried in
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A scintillation cocktail containing 25% Triton X-114 in xylene is considered for a broad range of scintsillation counting applications and gives good counting efficiencies for 3H and 14C.