Production of a new antiserum to arthropod molting hormone and comparison with two other antisera.

  title={Production of a new antiserum to arthropod molting hormone and comparison with two other antisera.},
  author={Cynthia Soumoff and Denis H.S. Horn and John D. O'connor},
  journal={Journal of steroid biochemistry},
  volume={14 5},
Abstract β-ecdysone 2-hemisuccinate was conjugated to thyroglobulin yielding 166 haptens per molecule of protein. The antiserum raised against this conjugate had a strong binding affinity for β-ecdysone and displayed reduced affinity for β-ecdysone analogs with altered hydroxyl functions along the sidechain. One exception was α-ecdysone which competed more effectively than β-ecdysone for antibody binding sites. Antiserum sensitivity toward these two hormones varied over a fifteen week interval… 
Radioimmunoassay and the characterization of crustacean ecdysteroids
The sensitivity of the RIA has permitted the monitoring of the rate of secretion of ecdysteroids by molting glands exposed to various in vitro conditions, and this latter approach will be of great utility in determining the nature and effect of molt regulators (inhibitors) present in other tissues.
Ecdysteroid titre and metabolism to novel apolar derivatives in adult female Boophilus microplus (Ixodidae)
The free ecdysteroid titre determined by radioimmunoassay in adult female Boophilus microplus showed a peak just prior to full engorgement and detachment of the ticks and decreased subsequently to a very low value, which suggests that they are of a fatty acyl ester nature.
Ecdysone metabolism and distribution during the pupal-adult development of Manduca sexta
The metabolism and distribution of endogenous ecdysone and injected [3H]ecdysone were studied during the pupal-adult development of Manduca sexta. Well-characterized antisera were used to detect and
A novel neuropeptide–endocrine interaction controlling ecdysteroid production in ixodid ticks
  • L. Lomas, P. Turner, H. Rees
  • Biology, Medicine
    Proceedings of the Royal Society of London. Series B: Biological Sciences
  • 1997
A new neuropeptide–endocrine interaction in the adult tick, Amblyomma hebraeum, is delineated that stimulates the synthesis of the moulting hormones, the ecdysteroids, which has some parallels with the known prothoracicotropic hormone (neuropeptides)–prothorACic gland endocrine axis of insects.
Evidence for the release of 20-hydroxyecdysone 25-glucoside by adult femaleParascaris equorum (Nematoda) in vitro
Whether ecdysone 25-glucoside production represents an active pathway of endogenous ecdysteroid metabolism, at least during maintenance in vitro is determined.
Ecdysone 20-monooxygenase activity in land crabs
Changes in ecdysone 20-monooxygenase activity at different stages of the molt cycle are not directly correlated to changes in ecdysteroid levels in the hemolymph.
Metabolism of Ecdysteroids During the Embryogenesis of Manduca Sexta
Abstract The combination of ion suppression, reverse phase high pressure liquid chromatography (IS-RPHPLC) and radioimmunoassay (RIA) employing two relatively non-specific, complimentary antisera was
Embryonic ecdysteroids in a mole crab,Emerita asiatica (Milne-Edwards)
Using radioimmunoassay (RIA) and high performance liquid chromatography (HPLC), the presence of a complex mixture of free and conjugated ecdysteroids is reported in the embryonated eggs of a mole
The occurrence of ecdysteroids in the cestode, Moniezia expansa.
Characterization of the free hormones by high-performance liquid chromatography monitoring fractions by radioimmunoassay, and by gas chromatography/mass spectrometry (selected ion monitoring) indicated the presence of ecdysone, 20-hydroxyecdysone and 20,26-dihydroxyECDysone.
A Developmental Analysis of Ecdysteroids During the Metamorphosis of Drosophila Melanogaster
Abstract A combination of ion suppression reverse phase high pressure liquid chromatography (RP-HPLC) and differential radioimmunoassay (RIA) was used to identify and quantify ecdysteroids from


A high affinity antiserum specific for the ecdysone nucleus.
Abstract The 22-hemisuccinate ester of α-ecdysone was conjugated to bovine thyroglobulin and used to raise an antiserum which has a greater affinity for α-ecdysone and an overall higher sensitivity
A radioimmunoassay for arthropod moulting hormones, introducing a novel method of immunogen coupling.
The antiserum has a greater affinity for ecdysone and 22-isoecdysone than for all other ecdysteroids and steroids tested.
The qualitative and quantitative determinations of ecdysones in tissues of the crab, Pachygrapsus crassipes, following molt induction.
From in vitro experiments, it is demonstrated that crab tissues have the ability to convert radiolabeled α- to β-ecdysone and the testis appears to have the greatest capacity for this conversion.
In vitro secretion and hydroxylation of α-ecdysone as a function of the crustacean molt cycle
The ability to hydroxylate the Y-organ secretory product to the predominant circulating form of the molting hormone is concentrated in the mitochondria-enriched fraction of the testes of the crabs, Pachygrapsus crassipes and Cancer antennarius.
Metabolism of some possible ecdysone precursors in Calliphora stygia.
A study has been made, using Calliphora stygia at the time of puparium formation, of the incorporation of a number of labelled sterols into β-ecdysone. [1α-3H]-Cholesterol and [4-14C]-cholesterol are
Arthropod molting hormones.
  • H. Hikino, Y. Hikino
  • Biology, Medicine
    Fortschritte der Chemie organischer Naturstoffe = Progress in the chemistry of organic natural products. Progres dans la chimie des substances organiques naturelles
  • 1970
This review article is an attempt to summarize recent developments in knowledge regarding the chemistry, synthesis and metabolism of the arthropod molting hormones, but will in the main exclude consideration of biological properties which have been frequently the object of excellent reviews.
The number and variety of known compounrjs between proteins and small molecules are increasing rapidly and make a fascinating story. For instance, there are the compounds of iron, which is carried in
An improved scintillation cocktail of high-solubilizing power.
A scintillation cocktail containing 25% Triton X-114 in xylene is considered for a broad range of scintsillation counting applications and gives good counting efficiencies for 3H and 14C.