Production of a monoclonal antibody as immunohistochemical marker on paraffin embedded tissues using a new immunization method.

@article{Pancino1990ProductionOA,
  title={Production of a monoclonal antibody as immunohistochemical marker on paraffin embedded tissues using a new immunization method.},
  author={Gianfranco Pancino and Eduardo Osinaga and W Vorauher and A Kakouche and Dominique Mistro and Colette Charpin and Alberto Roseto},
  journal={Hybridoma},
  year={1990},
  volume={9 4},
  pages={389-95}
}
This report describes a method for the production of murine monoclonal antibodies (MAbs) against cellular antigens preserved during formol fixation and paraffin embedding of human tissues in an attempt to select markers that would be useful in immunopathology. Hybridomas were prepared using spleen cells from mice immunized with cell suspensions obtained… CONTINUE READING