Production of a high-efficiency TILLING population through polyploidization.

  title={Production of a high-efficiency TILLING population through polyploidization.},
  author={Helen Tsai and Victor Missirian and Kathie J. Ngo and Robert K. Tran and Simon R W L Chan and Venkatesan Sundaresan and Luca Comai},
  journal={Plant physiology},
  volume={161 4},
Targeting Induced Local Lesions in Genomes (TILLING) provides a nontransgenic method for reverse genetics that is widely applicable, even in species where other functional resources are missing or expensive to build. The efficiency of TILLING, however, is greatly facilitated by high mutation density. Species vary in the number of mutations induced by comparable mutagenic treatments, suggesting that genetic background may affect the response. Allopolyploid species have often yielded higher… CONTINUE READING


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