• Chemistry, Medicine
  • Published in
    Applied and environmental…
    1984

Production of Solvents by Clostridium acetobutylicum Cultures Maintained at Neutral pH.

@article{Holt1984ProductionOS,
  title={Production of Solvents by Clostridium acetobutylicum Cultures Maintained at Neutral pH.},
  author={Robert Antony Holt and Gillian M. Stephens and James G. Morris},
  journal={Applied and environmental microbiology},
  year={1984},
  volume={48 6},
  pages={
          1166-70
        }
}
The formation of acetone and n-butanol by Clostridium acetobutylicum NCIB 8052 (ATCC 824) was monitored in batch culture at 35 degrees C in a glucose (2% [wt/vol]) minimal medium maintained throughout at either pH 5.0 or 7.0. At pH 5, good solvent production was obtained in the unsupplemented medium, although addition of acetate plus butyrate (10 mM each) caused solvent production to be initiated at a lower biomass concentration. At pH 7, although a purely acidogenic fermentation was maintained… CONTINUE READING

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