Production of Soluble and Active Transferrin Receptor-Targeting Single-Chain Antibody using Saccharomyces cerevisiae

@article{Hackel2006ProductionOS,
  title={Production of Soluble and Active Transferrin Receptor-Targeting Single-Chain Antibody using Saccharomyces cerevisiae},
  author={Benjamin J. Hackel and Dagang Huang and Jennifer C. Bubolz and Xin Xiang Wang and Eric V Shusta},
  journal={Pharmaceutical Research},
  year={2006},
  volume={23},
  pages={790-797}
}
This study describes the soluble production, purification, and functional testing of an anti-transferrin receptor single-chain antibody (OX26 scFv) using the yeast Saccharomyces cerevisiae. The yeast secretion apparatus was optimized by modulating expression temperature, the folding environment of the endoplasmic reticulum, and gene dosage. Secreted scFv was purified using immobilized metal affinity chromatography, and tested for binding and internalization into the RBE4 rat brain endothelial… CONTINUE READING

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