Production of Pentameric Cholera Toxin B Subunit in Escherichia coli

  title={Production of Pentameric Cholera Toxin B Subunit in Escherichia coli
  author={Farida Dakterzada and Ashraf Mohabati Mobarez and Mehryar Habibi Roudkenar and Mehdi Forouzandeh},
  booktitle={Avicenna journal of medical biotechnology},
Cholera toxin B subunit (CTB) has been extensively studied as an immunogen, adjuvant, and inducer of oral tolerance in many investigations. Production of CTB has been carried out in the bacterial, plant, insect and yeast expression systems. In this study the expression of the CTB containing a 6XHis-tagged was performed by Escherichia coli (E.coli) M15. The yield of purified pentameric recombinant CTB was about 1 mg/l. Western blot analysis demonstrated that the recombinant CTB was antigenically… CONTINUE READING
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