Production of Paracoccidioides brasiliensis exoantigens for immunodiffusion tests.

  title={Production of Paracoccidioides brasiliensis exoantigens for immunodiffusion tests.},
  author={Z P de Camargo and Carmelinda S. Unterkircher and Susana Campoy and Luiz R Travassos},
  journal={Journal of clinical microbiology},
  volume={26 10},
Growth curves of the yeast form of Paracoccidioides brasiliensis B-339 based on total and viable cell counts were determined. Crude culture filtrate antigens were obtained after 7, 10, 15, 20, 25, and 30 days of incubation. Different patterns of proteins were obtained by affinity chromatography on Sepharose 4B-immunoglobulin G complex made with immunoglobulin G from patients with paracoccidioidomycosis, with subsequent analyses by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and… CONTINUE READING
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