Production of Monoclonal Antibodies Using a Secretion Capture Report Web

Abstract

We describe a novel method for the production of monoclonal antibodies using a secretion capture report web (SCRW). Following HAT selection in bulk culture, individual hybridomas are encapsulated in biotinylated agarose drops. Antibody secreted by the hybridoma is captured within the agarose drop using an avidin bridge and biotinylated anti-mouse… (More)
DOI: 10.1038/nbt0895-787

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Cite this paper

@article{Kenney1995ProductionOM, title={Production of Monoclonal Antibodies Using a Secretion Capture Report Web}, author={John S. Kenney and Forest Gray and M A Ancel and J. F. Dunne}, journal={Bio/Technology}, year={1995}, volume={13}, pages={787-790} }