Production of Limonoids with Insect Antifeedant Activity in a Two-Stage Bioreactor Process with Cell Suspension Culture of Azadirachta indica

@article{VsquezRivera2015ProductionOL,
  title={Production of Limonoids with Insect Antifeedant Activity in a Two-Stage Bioreactor Process with Cell Suspension Culture of Azadirachta indica},
  author={Andr{\'e}s V{\'a}squez-Rivera and Diego Chicaiza-Finley and R. Hoyos and F. Orozco-S{\'a}nchez},
  journal={Applied Biochemistry and Biotechnology},
  year={2015},
  volume={177},
  pages={334-345}
}
Neem tree (Azadirachta indica) cell suspension culture is an alternative for the production of limonoids for insect control that overcomes limitations related to the supply of neem seeds. To establish conditions for cell growth and azadiracthin-related limonoid production, the effect of different sucrose concentrations, nitrate and phosphate in Murashige and Skoog (MS) medium, and the addition of one precursor and three elicitors was evaluated in shake flasks. The process was scaled up to a 3-l… Expand
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