Production of Human Tissue Plasminogen Activator in Transgenic Mouse Milk

@article{Gordon1987ProductionOH,
  title={Production of Human Tissue Plasminogen Activator in Transgenic Mouse Milk},
  author={K. Gordon and E. Lee and J. A. Vitale and A. Smith and H. Westphal and L. Hennighausen},
  journal={Bio/Technology},
  year={1987},
  volume={5},
  pages={1183-1187}
}
  • K. Gordon, E. Lee, +3 authors L. Hennighausen
  • Published 1987
  • Biology, Medicine
  • Bio/Technology
  • We set out to express an exogenous gene in the mammary epithelium of transgenic mice in the hope that the encoded protein would be secreted into milk. The promoter and upstream regulatory sequences from the murine whey acid protein (WAP) gene were fused to cDNA encoding human tissue plasminogen activator (t–PA) with its endogenous secretion signal sequence. This hybrid gene was injected into mouse embryos, resultant transgenic mice were mated, and milk obtained from lactating females was shown… CONTINUE READING
    191 Citations
    PRODUCTION OF HUMAN THERAPEUTIC PROTEINS IN TRANSGENIC ANIMALS
    • 4
    Transgenic animal bioreactors in biotechnology and production of blood proteins.
    • H. Lubon
    • Biology, Medicine
    • Biotechnology annual review
    • 1998
    • 35
    Expression of human blood clotting factor VIII in the mammary gland of transgenic sheep
    • 70
    Transgenic rabbits as bioreactors for the production of human growth hormone.
    • 44

    References

    SHOWING 1-10 OF 17 REFERENCES
    Production of transgenic rabbits, sheep and pigs by microinjection
    • 957
    • PDF
    Characterization and cloning of the mRNAs specific for the lactating mouse mammary gland.
    • 113