Production of Human Antibodies to Bee Venom Phospholipase A2 in Vitro

  title={Production of Human Antibodies to Bee Venom Phospholipase A2 in Vitro},
  author={William A. Held and M A Stucki and Christoph H. Heusser and Kurt Blaser},
  journal={Scandinavian Journal of Immunology},
Phospholipase A2 (PLA) is the major antigen of bee venom. Whereas individuals frequently stung by bees, such as bee keepers, show high levels of IgG4 anti‐PLA antibodies in serum, most patients sensitive to bee venom possess increased IgE anti‐PLA. We have established a culture system by which anti‐PLA antibodies can be induced in vitro. Peripheral blood mononuclear cells were stimulated in a first step with PLA and/or pokeweed mitogen (PWM). After 3 days of culture the cells were washed and… 
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Bee venom phospholipase A2‐specific T cell clones from human allergic and non‐allergic individuals: cytokine patterns change in response to the antigen concentration

These PLA‐specific Tcell clones could be classified according to the changes in the ratio of IL‐4/IFN‐γ production in response to increasing antigen concentrations, which may be a driving force for IgE or IgG formation resulting in allergy or immunoprotection.

Epitope-specific T cell tolerance to phospholipase A2 in bee venom immunotherapy and recovery by IL-2 and IL-15 in vitro.

The findings indicate that bee venom immunotherapy induces a state of peripheral tolerance in allergen-specific T cells, but not in specific B cells, suggesting the importance of microenvironmental cytokines leading to success or failure in immunotherapy.

Type I skin reactivity to native and recombinant phospholipase A2 from honeybee venom is similar.

Shift from Th2 to Th1 response in immunotherapy with venoms

Cytokines released from T lymphocytes regulate antibody production by B lymphocytes and releasability of mast cells and basophils and time pattern of cytokines secreted from PBMCs after stimulation with PMA and ionomycine was different than after stimulation through T-cell receptor/CD3 complex.

A system for stable indirect immobilization of multimeric recombinant proteins.



Isotypic and idiotypic characterization of anti-bee venom phospholipase A2 antibodies.

The anti-PLA response provides a human model to study the idiotypic regulation of isotypes in a defined system.

Bee keepers' IgG and IgE antibody responses to bee venom studied by means of crossed radioimmunoelectrophoresis

The immune response to honey bee venom in thirty‐seven bee keepers' sera was studied by several methods and significant amounts of IgG antibodies were found towards most bee‐venom components.

Antigen-induced in vitro antibody production in humans: a model for B cell activation and immunoregulation.

  • D. VolkmanH. LaneA. Fauci
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1981
An in vitro model of antigen induction of antigen-specific antibody synthesis in human peripheral blood mononuclear cells has been demontrated and should prove useful in exploring the mechanism of human B cell activation and immunoregulation.

Human IgE antibody synthesis in vitro: stimulation of IgE responses by pokeweed mitogen and selective inhibition of such responses by human suppressive factor of allergy (SFA).

This model can be used to compare the regulation of IgE synthesis in normal and atopic donors, and further experiments examining the production and action of this class-specific suppressive factor may have prognostic and therapeutic implications.

Serologic aspects of IgG4 antibodies. I. Prolonged immunization results in an IgG4-restricted response.

Evidence is provided that antigen-binding assays may even underestimate the contribution of IgG4 antibodies, because in contrast to IgG1 antibodies, IgG 4 antibodies act as monovalent antibodies in being unable to cross-link immunosorbent-bound antigen and radiolabeled antigen.

In vitro antigen-specific antibody response to the species-specific surface protein antigens of typhus group rickettsiae by human peripheral blood mononuclear cells: generation of an antigen-dependent suppressor T cell.

Prior exposure of T cells to high concentrations of SPA led to the generation of an antigen-dependent population of cells capable of suppressing the anti-SPA response when co-cultured with autologous PBMC and optimal SPA concentrations.

Characterization of a circulating subpopulation of spontaneous antitetanus toxoid antibody producing B cells following in vivo booster immunization.

The appearance of circulating B lymphocyte subpopulations responsible for the in vitro synthesis of IgG or IgM antitetanus toxoid antibody after in vivo booster immunization has been analyzed and indicates that multiple subsets of circulate B cells can be functionally defined in humans.

Effects of passive antibody in bee venom anaphylaxis.

These results represent the best available evidence for a direct role for IgG blocking antibodies in clinical protection against anaphylaxis occurring as a result of parenteral antigenic challenge as may be observed in penicillin and insect hypersensitivity.

The specificity of T-cell helper factor in man.

LMF is a nonspecific human T-cell helper factor which behaves like a polyclonal B-cell activator in the supernates of TT-stimulated human T cells but in the presence of specific antigen (TT) the antigen-specific B cell is preferentially triggered by LMF.