Production of Hevea brasiliensis transgenic embryogenic callus lines by Agrobacterium tumefaciens: roles of calcium

@article{Montoro2003ProductionOH,
  title={Production of Hevea brasiliensis transgenic embryogenic callus lines by Agrobacterium tumefaciens: roles of calcium},
  author={P. Montoro and W. Rattana and V. Pujade-Renaud and N. Michaux-ferri{\`e}re and Y. Monkolsook and R. Kanthapura and S. Adunsadthapong},
  journal={Plant Cell Reports},
  year={2003},
  volume={21},
  pages={1095-1102}
}
  • P. Montoro, W. Rattana, +4 authors S. Adunsadthapong
  • Published 2003
  • Biology, Medicine
  • Plant Cell Reports
  • A procedure has been established for Agrobacterium tumefaciens-mediated genetic transformation of Hevea brasiliensis embryogenic friable calli. Precultivation of tissues on a CaCl2-free maintenance medium dramatically enhanced the transient activity of the reporter gene, gusA encoding β-glucuronidase (GUS). The increase was first noticed in highly active cells (undifferentiated or/and embryogenic), in tissues precultured for 2–8 weeks. Beyond 8 weeks of preculture, GUS activity increased again… CONTINUE READING
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