Production of Hevea brasiliensis transgenic embryogenic callus lines by Agrobacterium tumefaciens: roles of calcium

@article{Montoro2003ProductionOH,
  title={Production of Hevea brasiliensis transgenic embryogenic callus lines by Agrobacterium tumefaciens: roles of calcium},
  author={Pascal Montoro and Wiparat Rattana and Val{\'e}rie Pujade-Renaud and Nicole Michaux-Ferri{\`e}re and Yupa Monkolsook and Reena Kanthapura and Saisunee Adunsadthapong},
  journal={Plant Cell Reports},
  year={2003},
  volume={21},
  pages={1095-1102}
}
  • Pascal Montoro, Wiparat Rattana, +4 authors Saisunee Adunsadthapong
  • Published in Plant Cell Reports 2003
  • DOI:10.1007/s00299-003-0632-7
A procedure has been established for Agrobacterium tumefaciens-mediated genetic transformation of Hevea brasiliensis embryogenic friable calli. Precultivation of tissues on a CaCl2-free maintenance medium dramatically enhanced the transient activity of the reporter gene, gusA encoding β-glucuronidase (GUS). The increase was first noticed in highly active cells (undifferentiated or/and embryogenic), in tissues precultured for 2–8 weeks. Beyond 8 weeks of preculture, GUS activity increased again… CONTINUE READING

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