Production of Haploid Saccharum spontaneum L. - Comparison of Media for Cold Incubation of Panicle Branches and for Float Culture of Anthers.

@article{Fitch1984ProductionOH,
  title={Production of Haploid Saccharum spontaneum L. - Comparison of Media for Cold Incubation of Panicle Branches and for Float Culture of Anthers.},
  author={Maureen M. M. Fitch and Paul H. Moore},
  journal={Journal of plant physiology},
  year={1984},
  volume={117 2},
  pages={169-78}
}
Means were sought to increase the frequency of haploid production from anther cultures of Saccharum spontaneum L. Excised preemerged panicle branches were incubated in modified Murashige-Skoog (MS), Nitsch (H), Gamborg (B5), and Chu (N6) media at 10 °C for 4 to 10 weeks prior to removal of the anthers for culturing at 27 °C. Anthers, either continuously cultured on liquid media or floated on liquid media 1 to 8 weeks prior to nurse culture, produced visible calli in 30 days. Nearly 1% of the… CONTINUE READING
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