Production of Bovine Embryos and Calves Cloned by Nuclear Transfer Using Mesenchymal Stem Cells from Amniotic Fluid and Adipose Tissue.

@article{Silva2016ProductionOB,
  title={Production of Bovine Embryos and Calves Cloned by Nuclear Transfer Using Mesenchymal Stem Cells from Amniotic Fluid and Adipose Tissue.},
  author={Carolina Gonzales da Silva and Carlos Frederico Martins and Tereza Cristina Cardoso and Elisa Ribeiro da Cunha and Heidi Christina Bessler and George Henrique Lima Martins and Ivo Pivato and S{\^o}nia Nair B{\'a}o},
  journal={Cellular reprogramming},
  year={2016},
  volume={18 2},
  pages={127-36}
}
The less differentiated the donor cells are used in nuclear transfer (NT), the more easily are they reprogrammed by the recipient cytoplasm. In this context, mesenchymal stem cells (MSCs) appear as an alternative to donor nuclei for NT. The amniotic fluid and adipose tissue are sources of MSCs that have not been tested for the production of cloned embryos in cattle. The objective of this study was to isolate, characterize, and use MSCs derived from amniotic fluid (MSC-AF) and adipose tissue… CONTINUE READING