Production of Alkaline Enzymes by Alkalophilic Microorganisms Part I . Alkaline Protease Produced by Bacillus No . 221 By Koki H 0 RIK 0

@inproceedings{H0RIK0SHI2008ProductionOA,
  title={Production of Alkaline Enzymes by Alkalophilic Microorganisms Part I . Alkaline Protease Produced by Bacillus No . 221 By Koki H 0 RIK 0},
  author={Koki H0RIK0SHI},
  year={2008}
}
  • Koki H0RIK0SHI
  • Published 2008
A crystalline alkaline protease was prepared from Bacillus No. 221 isolated from soil. The characteristic point of this microorganism is especially good growth in alkaline media. The enzyme was most active at pH 11.5--12 towards casein and was stable at pH values from 4 to 11 on 10 min incubation at 60°C. Calcium ion was effective to stabilize the enzyme especially at higher temperatures. The enzyme was completely inactivated by DFP and urea, but not affected by sulfhydryl reagent, EDTA, SLS… CONTINUE READING

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