Production of Abrus pulchellus ribosome-inactivating protein from seeds callus culture.

@article{Silva2003ProductionOA,
  title={Production of Abrus pulchellus ribosome-inactivating protein from seeds callus culture.},
  author={Andr{\'e} Luis Coelho da Silva and Ana Cec{\'i}lia G{\'o}es Horta and Renato de Azevedo Moreira and Leila Maria Beltramini and Ana P U Araujo},
  journal={Toxicon : official journal of the International Society on Toxinology},
  year={2003},
  volume={41 7},
  pages={841-9}
}
Ribosome inactivating proteins (RIPs) were isolated from callus culture that were established from seed explants of Abrus pulchellus. Cotyledon segments of immature seeds were inoculated in basal medium MS supplemented with different concentrations of auxin (2,4-D), citokinin (kinetin and BA) and sucrose in order to determine the best callus induction. A. pulchellus type 2 RIP (pulchellin) expression was monitored in callus cultures by RT-PCR and biological activity. The calli obtained after 35… CONTINUE READING