Production of 2-ketoisocaproate with Corynebacterium glutamicum strains devoid of plasmids and heterologous genes

@inproceedings{Vogt2015ProductionO2,
  title={Production of 2-ketoisocaproate with Corynebacterium glutamicum strains devoid of plasmids and heterologous genes},
  author={Michael Vogt and Sabine Haas and Tino Polen and Jan van Ooyen and Michael Bott},
  booktitle={Microbial biotechnology},
  year={2015}
}
2-Ketoisocaproate (KIC), the last intermediate in l-leucine biosynthesis, has various medical and industrial applications. After deletion of the ilvE gene for transaminase B in l-leucine production strains of Corynebacterium glutamicum, KIC became the major product, however, the strains were auxotrophic for l-isoleucine. To avoid auxotrophy, reduction of IlvE activity by exchanging the ATG start codon of ilvE by GTG was tested instead of an ilvE deletion. The resulting strains were indeed able… CONTINUE READING

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