Production of 10-Hydroxy-8(E)-Octadecenoic Acid from Oleic Acid Conversion by Strains of Pseudomonas aeruginosa

Abstract

Eighteen Pseudomonas aeruginosa strains were examined for their ability to convert oleic acid to produce 10-hydroxy-8(E)-octadecenoic acid (HOD), which was structurally confirmed by GC-MS, NMR, and FTIR. There were no substantial amounts of other new compounds found in the fermentation broths in addition to HOD and 7,10-dihydroxy-8(E)-octadecenoic acid (DOD). The results demonstrated that P. aeruginosa strains possessed varying levels of activity for producing HOD. Under the experimental conditions, strain NRRL B-14938 isolated from sheep manure was the best HOD producer exhibiting the highest HOD to DOD product ratio in the medium most suitable for purifying HOD. Using strain B-14938 as a model system for further characterization, optimum conditions for producing HOD were found to be at 26°C and pH 7.0 after 60 h of reaction time using a medium containing EDTA as a chelating agent. This study has identified a high-yielding P. aeruginosa strain and provided the reaction characteristics needed to develop a scale-up production process of HOD for testing its properties and potential new uses.

DOI: 10.1007/s00284-008-9226-5

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@article{Kuo2008ProductionO1, title={Production of 10-Hydroxy-8(E)-Octadecenoic Acid from Oleic Acid Conversion by Strains of Pseudomonas aeruginosa}, author={Tsung Min Kuo and Jenq-Kuen Huang and David P. Labeda and Lisa Wen and Gerhard Knothe}, journal={Current Microbiology}, year={2008}, volume={57}, pages={437-441} }