Production in Escherichia coli of a rat chimeric proinsulin polypeptide carrying human A and B chains and its preparative chromatography.

@article{Olmos1994ProductionIE,
  title={Production in Escherichia coli of a rat chimeric proinsulin polypeptide carrying human A and B chains and its preparative chromatography.},
  author={Jorge Olmos and Norberto Sotelo Cruz and Myriam Cant{\'u} S{\'a}nchez and Marcos Y. Lopez and Paulina Balb{\'a}s and Guillermo Gosset and Fernando Cano Valle and Francisco G Bol{\'i}var},
  journal={Journal of biotechnology},
  year={1994},
  volume={38 1},
  pages={89-96}
}
A pseudohuman proinsulin coding DNA sequence (MMRPI) carrying human A and B chains, was constructed via directed mutagenesis of a previously modified rat proinsulin cDNA (MRPI) and expressed as a tryptophan (Trp)LE-proinsulin fusion protein in Escherichia coli W3110. Expression of the hybrid gene was achieved by depletion of tryptophan from the medium. The heterologous fusion protein, accumulated as insoluble inclusion bodies within the cell, was obtained by differential centrifugation and then… CONTINUE READING

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