Production and secretion of recombinant Leuconostoc mesenteroides dextransucrase DsrS in Bacillus megaterium.

@article{Malten2005ProductionAS,
  title={Production and secretion of recombinant Leuconostoc mesenteroides dextransucrase DsrS in Bacillus megaterium.},
  author={Marco Malten and Rajan Hollmann and W Deckwer and Dieter Jahn},
  journal={Biotechnology and bioengineering},
  year={2005},
  volume={89 2},
  pages={206-18}
}
Leuconostoc mesenteroides dextransucrase DsrS was recombinantly produced in Bacillus megaterium and exported into the growth medium. For this purpose a plasmid-based xylose-inducible gene expression system was optimized via introduction of a multiple cloning site and an encoded optimal B. megaterium ribosome binding site. A cre mediating glucose-dependent catabolite repression was removed. Recombinant DsrS was found in the cytoplasm and exported via its native leader sequence into the growth… CONTINUE READING