Production and secretion of biologically active recombinant canine growth hormone by Pichia pastoris.

@article{AscacioMartnez2004ProductionAS,
  title={Production and secretion of biologically active recombinant canine growth hormone by Pichia pastoris.},
  author={Jorge {\'A}ngel Isidro Ascacio-Mart{\'i}nez and Hugo Alberto Barrera-Salda{\~n}a},
  journal={Gene},
  year={2004},
  volume={340 2},
  pages={261-6}
}
Production of recombinant canine (Canis familiaris) growth hormone (rCFGH) by two expression systems, methanol utilization slow (Muts) and methanol utilization plus (Mut+) based on Pichia pastoris. Led by the Saccharomyces cerevisiae alpha-mating type signal sequence (SS), the hormone was secreted into the culture medium in its mature and active form. The level of total proteins secreted into the medium achieved at 25 ml working volume using Erlenmeyer flasks was approximately 40 and 15 microg… CONTINUE READING