Production and purification of a recombinant human hsp60 epitope using the cellulose-binding domain in Escherichia coli.

@article{Shpigel1998ProductionAP,
  title={Production and purification of a recombinant human hsp60 epitope using the cellulose-binding domain in Escherichia coli.},
  author={Etai Shpigel and Dana Elias and Irun R. Cohen and Oded Shoseyov},
  journal={Protein expression and purification},
  year={1998},
  volume={14 2},
  pages={185-91}
}
The heat shock protein hsp60 plays a functional role in insulin-dependent diabetes mellitus. The hsp60 epitope p277 (aa 437-aa 460) is effective in vaccinating mice against diabetes. A synthetic peptide gene (p277) that encodes the human hsp60 epitope was cloned to the 3' end of the cellulose-binding domain gene (cbd). CBD-p277 was overexpressed in… CONTINUE READING